Parece que o salto de linha está sendo ignorado quando o dfrac é colocado no final da lista. Por que isso acontece?
\documentclass[10pt]{article}
\usepackage{amsmath}
\usepackage{amssymb}
\usepackage{amsthm}
\usepackage{enumitem}
\renewcommand{\labelenumii}{\textbf{\Alph{enumii}.}}
\setlength{\lineskip}{4pt}
\newenvironment{myenum}
{\begin{enumerate}[labelsep=2.5mm, align=left,leftmargin=*]
\setlength{\itemsep}{1pt}
\setlength{\parskip}{0pt}
\setlength{\parsep}{0pt}}
{\end{enumerate}}
\begin{document}
\begin{enumerate}
\item blahblah
\begin{myenum}
\item $4.25 - c > 200$
\item $4.25 + c \leq 200$
\item $\dfrac{c}{4.25} > 200$ %%% when this is placed at the end of the list, the spacing before it gets reduced
\item $4.25c \leq 200$
\item $4.25c > 200$
\end{myenum}
\end{enumerate}
\end{document}